DNA

Part:BBa_K2100066

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pEXPR EGSH: mKate

This part is an expression vector containing PonA-inducible promoter EGSH and red fluorescent protein mKate. This part is used to see the induction of the EGSH promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal BglII site found at 115
    Illegal BamHI site found at 663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1334
    Illegal SapI.rc site found at 646
    Illegal SapI.rc site found at 716


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Parameters
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